HSC phenotype database





This database collates information on the hematopoietic phenotypes of knock-out mice as collected from the scientific literature. Entries have been curated by members of the Goodell Lab. For the most part, we have only included genes whose role in hematopoiesis has been tested by transplantation of bone marrow or stem cells from knockout mice into recipients. We encourage you to add your favorite genes and phenotypes into the database.


Rossi L, Lin KK, Boles, NC, Yang, L, King, KY, Jeong, M, Mayle, A, and MA Goodell (2012) Less is more: unveiling the functional core of hematopoietic stem cells through knockout mice. Cell Stem Cell.

Comments and suggestions are welcome! Please send them to us at HSCs@bcm.edu


 

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A Pre-transplantation defect H Apparently normal
B Altered FL hemo I Increased engraftment
C Transplantation assays performed J Change in phenotypic HSC number
D Severe in vivo defect K Change in cell cycle activity
E Moderate in vivo defect L Criteria used for HSC phenotype definition
F Mild in vivo defect M Cell extrinsic effect
G Defect but only after second or third txt N Cancer
NC No change ND Not done

 

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GENEPROTEIN TYPETYPE OF MOUSEABCDEFGHIJKLMNCOMMENTSPMID
Cdkn1a (p21)CDK inhibitorKOYESYES+Lin-No homing defects of KO Sca1+Lin- BM cells; Increased short-term progenitor pool, no change in absolute HSC number; no difference in PB count or BM cellularity10710306
Cdkn1b (p27)CDK inhibitorKOYESYES+NCRadioprotection assay: better even at the 4th BMT; competitive BMT: KO BM out-compete WT cells; No apparent homing difference; Increased short-term progenitors with normal HSC numbers, increased repopulating abilities11062534
Cdkn1c p57(kip2)CDK inhibitorcKO (Mx1Cre)YESYES-+KSL CD150+ & KSL CD34-BM and BM KSL transplant21885021
Cdkn1c p57(kip2)CDK inhibitorKOYESYESNCNCKSLDefect in tertiary FL transplant21885020
Cdkn2a/Cdkn2d (p16Ink4a/p19Arf)CDK inhibitordKOYESYESSlightly increased serial repopulating ability; better survival to the 3rd round)15692066
cdkn2c (p18)CDK inhinitorKOYESYES++KSL CD34-Increased repopulating ability in primary and secondary transplants, increased HSC frequency15122268
Cdkn2d (p19Arf)CDK inhibitorKOYESYESNCKSLuse ArfGFP mice that tested Arf expression in KLS population: no p19 expression in KLS.15692066
CebpaTFcKO (Mx1Cre)YESYESYESYES+NCKSLYESIncreased repopulating ability in primary and secondary transplants; loss of granulopoiesis in spleen as well, so not a specific defect in exit from BM; phenotype linked to increased Bmi1 expression; most mice died within weeks, a few mice survived several months, but did not develop AML; Absence of mature grans in PB, myeloid diff. block 15589173
Cited2TFKOYESYESYESYESNCFL KSLReduced colony formation; Reconstitution potential significantly reduced; Decreased fetal HSC pool; Fetal liver progenitors decreased and defective17644732
cMplTPO receptorKOYESYESYESEither decreseaed HSC or decreased ability of MPP to proliferate; Decreased numbers of GMP and MEPs17347409
cMplTPO receptorKOYESYESDecreased CFU-S and progenitors9448308
cMybTFcKO (Mx1Cre)YESYESYES--KSL Flk2- Thyl+Depleted HSC pool, an abolishment of self-renewal, a slightly reduced proliferative capacity, and a complete loss of colony growth and multi-lineage differentiation on methylcellulose.19955420
CrebbpTranscription co-activatorKOYESYESYES-KSL CD34-YESDistinct role of CBP-/- Self-renewal defect, p300-/- differentiation defect. P300-/- decrease HSC with age; decreased colony formation from P300-/- ; Some mice evenutally succomb to lymphoma.12397173
Csf3rReceptor (CD114)KOYESNDDefective granulopoiesis with chronic neutropenia and a decrease in mature myeloid, neutrophil apoptosis increased; decreased in vitro colonies8934575
Ctnnb1Adhesion junction complexcKO (Vav-cre)YESYESNCKSL FLK2-Decreased repopulating ability; BCL-ABL infected Ctnnb1 deficient cells develop ALL instead of CML18068630
Ctnnb1Adhesion junction complexcKO (Mx1Cre)YESYESNCKSL14718516
Cxcl12Cytokine aka SDF1 aka PBSFKOYESYESYES-Repopulating unitsFailure of HSC to home to bone marrow during development; no BM seeding; Decreased HSCs by E18.512397173
Cxcr4chemokinecKO (Mx1Cre)YESYESYES-+KSL CD34- or lower-SPProgenitor expansion, reduction in HSC quiescence; reduced in vitro colony formation; Pre-txt: Reduced B cells in BM, increased myeloid in PB17174120
Cxcr4receptor for Cxcl12KOYESYESNC+KSL CD48- Flk2-Increased engraftment activity when deleted after homing18378795
CyclinD1/D2/D3cyclinTriple KOYESYESYESYES--FL KSL IL7Ra-Studied FL; decreased number of all progenitors (CMP, CLP, MEP, GMP, HSC) compartment in FL; Pre txt: Anemic embryos plus cardiac defect15315760
DicerRNA binding proteincKO (Osx-Cre)YESYESYESNC+KSL CD150+CD48-YESYESSevere phenotype: increased HSC proliferation and apoptosis; later development of MDS and leukemia. Increased apoptosis in HSC and KSL. Osteo-progenitor specific: reciprocal transplantation of WT cells into KO developed leukopenia and MDS; Pre txt: Impaired osteoblast differentiation; Leukopenia; MDS; develop myeloblastic tumor20305640
Dmap1transcription regulatorshRNA K-downYESYESReduced repopulation and in vitro proliferation; growth retardation and reduced CFUs20387133

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