HSC phenotype database





This database collates information on the hematopoietic phenotypes of knock-out mice as collected from the scientific literature. Entries have been curated by members of the Goodell Lab. For the most part, we have only included genes whose role in hematopoiesis has been tested by transplantation of bone marrow or stem cells from knockout mice into recipients. We encourage you to add your favorite genes and phenotypes into the database.


Rossi L, Lin KK, Boles, NC, Yang, L, King, KY, Jeong, M, Mayle, A, and MA Goodell (2012) Less is more: unveiling the functional core of hematopoietic stem cells through knockout mice. Cell Stem Cell.

Comments and suggestions are welcome! Please send them to us at HSCs@bcm.edu


 

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A Pre-transplantation defect H Apparently normal
B Altered FL hemo I Increased engraftment
C Transplantation assays performed J Change in phenotypic HSC number
D Severe in vivo defect K Change in cell cycle activity
E Moderate in vivo defect L Criteria used for HSC phenotype definition
F Mild in vivo defect M Cell extrinsic effect
G Defect but only after second or third txt N Cancer
NC No change ND Not done

 

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GENEPROTEIN TYPETYPE OF MOUSEABCDEFGHIJKLMNCOMMENTSPMID
MatkPTKKOND+SP-KSLHSC is examined as a control for other immune cells, looking in the spleen. Increased HSC pool; deficiency of the spleen cells in making IFN-g16574955
Mcl1anti-apoptosis proteincKO (Mx1Cre)YESYESYES-KSLTotal loss of HSC compartment; KO BM generate fewer CFU; increased apoptosis in all progenitor compartments in vitro; SCF induces expression of MCL1; Pre txt: Died 12-21 days post deletion; anemic and BM hypocellular15718471
Mds1-Evi1 (ME)PR domain-containing zinc-finger TFKO (exon1 LacZ KI)YESYESYES-+KSL CD150+ CD48-HPP-CF assay: 1/3 of cells in ME KO compared to WT BM; moderate short term engraftment; Pre txt: Increased leukocytes and fewer platelets21666053
MEF/ELF4TFKOYESYESYES+-KSL CD34- Flk2-& SP-KSLInreased HSC compartment with increased quiescence. Large number of HSCs correlates with faster 5FU recovery. KO cells are more radio-resistant. And see PMID 19128791 for dKO with p5316530702
Mef2cTFcKO (Mx1Cre)YESYESNCKLS Flk2-defect in lymphoid differentiation19169261
MLL1Chromatin modifier; trithorax groupKO and cKO(Vav-cre)YESYESYESPre txt: Decreased HSC number in FL; Adult hematopoiesis normal until transplant18371367
MLL1Chromatin modifier; trithorax groupcKO (Mx1Cre)YESYES+Severe defect in deleted animals before and after transplantation18371366
Msi2RNA binding proteinGene trapYESYES-Severe defects in HSC and progenitors21613258
MybTFENU Hypomorph in transactivation domainYESYESYES++KSLIncrreased contribution to PB but skewed toward myeloid; more HSC. Same patterns in 2ndary txt; increased HSC pool, differentiation defects; unchanged BM cellularity, 2-10x fewer splenocytes and thymocytes in BM, incr megakaryocytes in BM; in vitro fewer erythrocytes; pre txt: Thrombocytosis, megakaryocytosis, anemia, lymphopenia, no eosi15691758
Myst3co-activator and HATKOYESYESYESYES-FL KL AA4.1+Decreased fetal HSC pool and repopulating ability16651658
Myst3co-activator and HATKOYESYESYESYES-FL KSLDecreased fetal HSC pool and repopulating ability16702405
NecdinTFKOYESYES++KS CD34-Slightly reduced stress recovery19770359
NKAPTranscriptional repressorcKO (Mx1Cre)YESYESYES--CD150+CD41-CD48-Rapid loss of HSC with apopotosis and decreased proliferation; Increased p21Cip1/Waf1 and p19Ink4d; Die within 2 weeks20610818
Nr4a1/Nr4a3TFdKOYESND+KSL CD34-Flk2-YESIncreased LT-HSC phenotype and expansion of myeloid progenitors --> AML development; Pre txt: Hepato-splenomegaly, lymph- adenopathy, anemia and thrombocytopenia17515897
Numb/Numbladaptor proteinDouble cKO (Mx1cre)YESYESNCNCKSLNormal HSC; no change in CMP and CLP17513721
Opn (Spp1)Cell surface glycoproteinKOYESYESYESYES+NCKSL & KSL CD34-YESTransplant of KO BM cells showed significantly higher engraftment; In serial transplantation, OPN-/- HSCs lose advantage of numbers by 2ndary transplant; signficantly lower number of CFC in BM of KO; microenvironment provided by Opn-/- mice able to support greater # of primitive cells in a stroma-dependent manner; Increased HSC pool; incre15928197
Opn (Spp1)Cell surface glycoproteinKOND++KSLTransplants of WT HSC into KO exhibit decreased survival after 3 weeks, KSL exhibited increased proliferation and number; Defective homing of WT HSCs in KO BM; exogenous Opn suppresses proliferation and differentiation of primitive HPCs in vitro,15845900
p300transcription coactivatorKOYESYESYES-FL KSL CD34-Slightly less abundant FL progenitors assayed as CFC in methylcellulose; Hets show small reduction in self-renewal; impaired differentiation of KO12397173
Pbx1TF (TALE homeodomain)cKO (Tie2-cre & Mx1cre)YESYES-+KSL CD150+ CD48-Reduced TGFb signalling18462698
Pbx1TF (TALE homeodomain)KOYESYESYESYES-FL KSL AA4.1+Decreased repopulating ability of fetal HSC, slightly decreased fetal hsc (p=0.07); E15.5 KO embryos showed profound anemia11468159
Pcgf2 (mel18)polycomb group ring fingerKOND+-KSL Flk- & KSL CD34-Increased HSC self-renewal, percentage of HSC increased but absolute number did not,15183898
Pik3r1 / Pik3r2lipid kinaseHet dKOYESYESDecreased fetal progenitor pool and decreased repopulation ability; decreased KitL-induced and Akt induced prolif, reduced LT-repopulation in competitive repop assay16239435

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