HSC phenotype database





This database collates information on the hematopoietic phenotypes of knock-out mice as collected from the scientific literature. Entries have been curated by members of the Goodell Lab. For the most part, we have only included genes whose role in hematopoiesis has been tested by transplantation of bone marrow or stem cells from knockout mice into recipients. We encourage you to add your favorite genes and phenotypes into the database.


Rossi L, Lin KK, Boles, NC, Yang, L, King, KY, Jeong, M, Mayle, A, and MA Goodell (2012) Less is more: unveiling the functional core of hematopoietic stem cells through knockout mice. Cell Stem Cell.

Comments and suggestions are welcome! Please send them to us at HSCs@bcm.edu


 

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A Pre-transplantation defect H Apparently normal
B Altered FL hemo I Increased engraftment
C Transplantation assays performed J Change in phenotypic HSC number
D Severe in vivo defect K Change in cell cycle activity
E Moderate in vivo defect L Criteria used for HSC phenotype definition
F Mild in vivo defect M Cell extrinsic effect
G Defect but only after second or third txt N Cancer
NC No change ND Not done

 

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Records: 503 | Pagination Enable / Disable | Download as CSV

GENEPROTEIN TYPETYPE OF MOUSEABCDEFGHIJKLMNCOMMENTSPMID
Potb1Telomere binding proteinKOYESYESYES-+KSLAged KO presented hallmarks of telomere dysfunction; Low LTC-IC colonies and Low CFU colonies; increased apoptotic BM and peripheral blood; KO failed to engraft SCID mice; Pre-txt: Low bone marrow cellularity by 6 month old21948176
Prdm16TFKOYESYES-KSL CD34-Flk2- & KSL CD150+Flk2-Increase in HSC apoptosis and cycling21343612
PrnpMembrane glycoproteinKOYESYESDecreased serial repopulating ability; LT-HSC surface marker16467153
Ptenphosphoinositide phosphatasecKO (Mx1Cre)YESYES++KSL Flk2-YESDecreased HSC pool and repopulating ability; increased HSC mobilization; increased CLP post induced PTEN deletion; severe depletion of BM KSL; Myeloproliferative disorder/AML; ~30 days post deletion16633340
Pten/b-cateninWnt and Akt pathwaysdouble cKO (Mx1Cre)YESND+KSL CD34-YESNo change in frequency of CMP or CLP pop. increased KLS pop, but decreased KLSca1- pop; Developed leukemia 6 weeks post deletion21890648
PU.1TFcKO (Mx1Cre)YESYESYES-SP KSLDecrease in FL HSC by transplantation; block myelomonocytic differentiation in CMP/GMP;15914556
Puma (Bbc3)BCL2 binding componentKOYES+KSL CD34- CD150+HSC number NC before irradiation; after irradiation, decreased apoptosis and more efficient DNA repair and significantly increased HSC number relative to controls20177048
Rac1GTP binding proteincKO (Mx1Cre)YESYESNCLKDecreased homing leading to decreased repopulating ability16025125
rac1/rac2GTP binding proteindKOYESYES-NCLKIncreased HSC mobilization; neutrophilia16025125
rac2GTP binding proteinKOYESYES-LKDecreased repopulating ability15749928
Rae28Polycomb complexKOYESYESYES-NClimiting dilutionDecreased primary and serial txt ability; perinatal lethal, FL were characterized; FL cells also showed reduction in LTC-IC, methylcellulose, and CFU-S assay11901201
Rbcell cycle suppressorcKO (Mx1-Cre)YESYESpreamble of the next paper where they introduced the deletion in the mice prior transplantation and found the microenvironment effect to myeloproliferative disease16754850
Rbcell cycle suppressorcKO (Mx1Cre)YESYESYESNCKSL CD34-YESYESLoss of trabecular bone; increased spleen weight; increased KSL and Lin-Kit+Sca- BM cells; Myeloproliferative-like disease; synergistic interaction between Rb-/- microenvironment and Rb-/- myeloid cells: transplant recipients with this combination died within 11 weeks; 'Anemic after deletion; thrombocytosis; pan-leukocytosis17574022
Rbm15/Ott1TFcKO (Mx1Cre)YESND+KSLincreased KSL and myeloid progenitor pool and expansion of myeloid compartment; Decreased Lymphocytes17376872
Runx1TFcKO (Mx1Cre)YESYESIncreased HSC pool and increased quiescence, differentiation defects; increased CFU-meg, myeloid, mixed; elevated colony replating capacity up to 2 months14966519
Runx1TFcKO (Mx1Cre)YESYES+-SP KSL CD34-Found increased KO cells in the BM after competitive transplant, did not test PB18354160
RxraTF (nuclear hormone)cKO (Mx1Cre)YESYESNCLS25%-40% incr colony formation16888087
Sca1GPI surface proteinKOYESYESCFUHoming defect; no self renewal defect when tested by 5FU treatment of chimeras; Sca1 KO may have defective IFN response (Essers 2010)15963860
Sca1GPI surface proteinKOYESYESYESCFULymphocytosis, thrombocytopenia pre txt12393491
SclTFcKO (Mx1Cre)YESYESYES-KSLSlightly decreased repopulating ability; Pre txt: LSK increase in number of LK cells in SCL-deleted mice14726374
SclTFcKO (Mx1Cre)YESYESYES-KSLScl null mice die during embryogenesis from severe hematopoietic defects. cKO show very mild phenotype; 3-4X increase in number of LSK and LK cells in SCL-deleted mice12540851
Sfrp1Soluble proteinKOYESYESNC-KSL CD34-Flk2, CD150+CD48-Sfrp1 is required to maintain HSC homeostasis through extrinsic regulation of ?-catenin; No intrinsic effect of Sfrp1 loss on the number of HSCs or their ability to engraft irradiated recipients19664990

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