HSC phenotype database

This database collates information on the hematopoietic phenotypes of knock-out mice as collected from the scientific literature. Entries have been curated by members of the Goodell Lab. For the most part, we have only included genes whose role in hematopoiesis has been tested by transplantation of bone marrow or stem cells from knockout mice into recipients. We encourage you to add your favorite genes and phenotypes into the database.

Rossi L, Lin KK, Boles, NC, Yang, L, King, KY, Jeong, M, Mayle, A, and MA Goodell (2012) Less is more: unveiling the functional core of hematopoietic stem cells through knockout mice. Cell Stem Cell.

Comments and suggestions are welcome! Please send them to us at HSCs@bcm.edu


A Pre-transplantation defect H Apparently normal
B Altered FL hemo I Increased engraftment
C Transplantation assays performed J Change in phenotypic HSC number
D Severe in vivo defect K Change in cell cycle activity
E Moderate in vivo defect L Criteria used for HSC phenotype definition
F Mild in vivo defect M Cell extrinsic effect
G Defect but only after second or third txt N Cancer
NC No change ND Not done


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Shp2 (Ptpn11)PTKcKO (Mx1Cre)YESYESYES-+KSL CD34-Rapid loss of HCs and multi lineage defects; Shp2 regulates c-kit via Gata2; Pre txt: Loss of BM cellularity and of HSCs21450902
Sipa1 (Spa-1)GAPKOYESND+KSLYESIncreased HSC pool and myeloid leukemia; myleoid leukemia (resembling CML), minor group with MDS-like symptoms; Pre txt: Anemia, leukocytosis, marked splenomegaly, extramedullary hematopoiesis after 1 year12892713
Sirt1chromatin modifier (HDAC)KO and hetNDNC+KSLDecreased numbers of HSCs and increase in cycling; increased proliferation in KO in VITRO culture18184747
Skp2F-box unit of the SCF E3-ligaseKOYESYESYES+-KSL IL7R? CD34?Flk2-Enhanced HSC quiescence, increased HSC pool size, and maintenance; Pre txt: BM cellularity decreased 30%. LSK cells increased21502543
SlugTFKOYESYESNCKSL CD34-Flk2 & CD150+CD48- & KSL EPCR+Huge increase in primitive HPC and radioprotection ability and repopulation; CFU-S increased, KSL expansion during in vitro culture, Increased spleen KSL20032500
Smad4TFcKO (Mx1Cre)YESYESYES-NCKSLDecreased repopulating ability; Pre txt: Reduction in RBC and hemoglobin17353364
Smad5TFcKO (Mx1Cre)YESYESNCKSL CD34-Normal HSC in adult; KSL cells show similar response to Tgfb1 as T16896158
SmoReceptor; HH signalingcKO (Mx1Cre)YESYESNCKSL CD150+ CD48- & KSL CD34- Flk2-Normal HSC19497283
SmoReceptor; HH signalingcKO (Mx1Cre)YESYESNCKSL & KSL CD34- Flk2-Normal HSC19497284
Sox17TFKOYESYESYESYESYESNC-KSL Tie2Decreased fetal HSC pool and hematopoietic cells; no impact of deletion in adult HS; Pre txt: Decreased hematopoiesis in FL and neonates17655922
Stat5TFcKO (Mx1Cre)YESYES-+KSLNOCan use as host for WT cells with or without irradiation: KO cells cannot compete with WT19258595
Stat5TFN-terminal truncated protein expressed in Stat5ab null fetal liverYESYESYESFL txt; Decreased repopulating ability17976521
Stat5TFSTAT5ab dKOYESYESYESYESNCKSL & SPTer119+ cell expansion, T cell accumulation, and signs of autoimmune disease; Pre txt: Reduced BM cellularity; reduced WBC, lymphocyte count, and hemotocrit; FL: FL transplant normal without competitor, but fail to engraft with competitor BM11781228
Stat5TFStat5ab/Rag2 triple KOYESYESDecreased repopulating ability12393407
Stat5TFStat5ab dKOYESYESKSL & KSL CD34- Flk2-2ndary transplant showed severely defective engraftment; KO KSL failed to respond to SCF and IL3 in vitro12393407
Tcea1Transcription elongation factor S-IIKOYESYESYESNCFL KSLDecreased expansion of fetal HSC16581793
Tet2methylcytosine dioxygenaseKO, cKO (Mx1Cre and Vav-cre)YESYESYES+KSL and KSL-CD150YESBM normal at 6 weeks, but ~20 weeks, massive increase in progentiors. Marked advantage in competitive Txt experiments. Remarkable re-plating phenotype. Mice eventually develop CMML-like disease. Het phenotype.21723200
Tet2methylcytosine dioxygenaseGene trap and cKO (Mx1Cre)YESYESYES+KSL and KSL-CD150YESIncrease in HSC and progenitors pre-Txt and post-txt. Het phenotype. Aberrant myeloid and lymphoid differentiation. With age, KO mice develop myeloid malignancies reminiscent of human CMML21723201
Tet2methylcytosine dioxygenaseKOYESYESYES+KSLKO have higher KSL progenitors, have higher engraftment after txt in all lineages, and in vitro show enhanced differentiation into moncytes (in addition to sustained preservation of progenitors).21873190
Tgfb2cytokineKOYESYESYESYES-KSLDecreased serial repopulating ability from fetal HSC, decreased HSC in adult mice; Het KSL response to cytokine-related growth (flt3, tpo and scf) in CFC assay is reduced; Pre txt: Slightly reduced bone marrow cellularity14707111
Tgfbr1ReceptorcKO (Mx1Cre)YESYESNCKSL CD34-Normal HSC; KO HSCs non-responsive to Tgf-b treatment in vitro12842983
Tnfrsf1a (p55)ReceptorKOYESYESDecreased repopulating ability10562323